Research Methodology and Procedure:
1. Plant collection and processing.
The plant samples used in preparing the extractions obtained commercially or collected from sites locally.
2. Preparation of plant leaf extract.
Extract the plant leaves by different methods by aqueous and organic solvent and the resulting extract was used for AgNps synthesis.
3. Preparation of silver nanoparticles.
Silver nitrate (AgNo3) used as a precursor for synthesis AgNps. The prepared plant extract is incubated with desired concentration of AgNO3 solution in a fixed ratio.
4. Identify the Functional groups of organic compounds in plant extract by Fourier transform infrared spectroscopy (FTIR).
FTIR technique is used to identify the possible biomolecules present in plant extracts involved in reduction and capping of Ag+ ions leading to efficient stabilization of the synthesized AgNPs.
5. Characterization of silver nanoparticles using different techniques.
The color changing of the mixture with the passage of time which is the first visual indication of the synthesis of AgNPs. Bio-reduction of silver ions by phytochemicals observed by a change in color then The synthesis of AgNPs is initially confirmed by UV–Visible spectrophotometry.
6. UV-Vis spectral analysis of the AgNps by UV-Vis spectrophotometer:
UV-vis is the most widely used technique for the structural characterization of nanoparticles, so the sizes of the synthesized nanoparticles were provisionally predicted on the basis of UV-vis spectrum. which gives different distinctive peaks of absorptions between 420 to 460 nm, due to the surface plasma resonance (SPR) of the AgNPs produced. After that further characterization of AgNPs is carried out with other analytical techniques to find out the various characteristics of synthesized AgNPs.
7. Analysis of silver nanoparticles by Transmission Electron Microscopic (TEM).
Transmission Electron Microscopy performed for characterizing size and shape of biosynthesized silver nanoparticles.
8. In-vitro anti-fungal activity of silver nanoparticles:
To investigate the antifungal activity of synthesized silver nanoparticles will use the agar-well diffusion method against different A§spergillus species.
9. Scanning Electron Microscopic Analysis:
Use of scanning electron microscopy to identify morphological damage on tested fungi due to AgNps application.