Radioprotection by a Novel Synthesized Chromone Derivative Pre and Post Irradiation-Induced Intestinal Inflammation in Male Albino Mice OMAMA E

Radioprotection by a Novel Synthesized Chromone Derivative Pre and Post Irradiation-Induced Intestinal Inflammation in Male Albino Mice

OMAMA E. Elshawi1, Asmaa I. Nabeel2*
1Clinical Health Radiation Research Department, National Centre for Radiation Research and Technology, Atomic
Energy Authority, PO Box 29 Nasr City, Cairo, Egypt
2Biochemistry Lab. Chemistry Department, Faculty of Education, Ain Shams University, Egypt
Abstract:
Benzopyran based compounds is a huge family containing chromones, coumarins and flavones. These compounds are mainly naturally extracted and some of them are synthetic. In a previous study, a new Chromone (Ch) derivative ;( 2E)-2- (4-oxo-4H-chromen-3-yl) methylene amino-4- nitrobenzoic acid was designed and characterized.
For acute, herein, different doses (0, 200, 250, 300, 350, 400 and 450 mg/kg) of the prepared chromone derivative was administered intraperitoneally to the different groups of mice. Signs of toxicity and possible death of animals were monitored for 24 hrs. The median lethal dose (LD50) was calculated and the dose of 43.5mg/kg was used to evaluate the therapeutic and prophylactic roles of the synthesized Chromone derivative in modulating the inflammatory alterations in the mice’s small intestine following gamma irradiation exposure. Toxicological evaluation showed no hepatic toxicity with marginal renal toxicity within the range of the applied dose (43.5 mg/kg).

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In comparison to irradiated animals, the protective effect of Ch compound was also observed throughout three weeks as there was marked reduction MDA and NO levels. Similarly, there were significant reductions in NFkB and caspases-3 activities as well as Cox-2, iNOs, TNF-? and IL-6 in the intestinal tissue of treated mice.
In conclusion; the Ch derivative protects the intestine against irradiation-induced inflammatory responses via its enzymatic inhibitory effect towards Cox-2 and iNOs. Ch compound was able to significantly inhibit NFkB activation and reduce the production of the measured pro inflammatory cytokines.