In conclusion, henna
has many traditional and commercial uses, the most common being as a dye for
hair, skin and fingernails, as a dye and preservative for leather and cloth,
and as an anti-microbacterial. The skin has an comples flora which can
breakdown the integrity of the skin when the immune defense is compromised. We have
conclude that Lawsonia Inrmis posses high antimicrobacterial activity against
P.eruginosa. By using cream or soaps incorporating henna active ingredients may
be great advantage for hygiene purposes for both physicians and patients in hospitals especially in intensive care
units or infectious disease units where immuno-compromised patients are treated.
L. inermis should be emphasized for the control of various diseases.
Henna imbibing a tremendous potential deserves a special attention of the
scientific fraternity to emerge as a milestone for medical science of this
millennium due to its various medicinal uses. Further evaluation needs to be
carried out on L. inermis L. in order to explore the concealed areas and their
practical clinical applications, which can be used for the welfare of the
mankind. Henna is a traditional
cosmetic agent and is used worldwide. It is used worldwide not only as a
cosmetic agent to stain the hair, skin and nails but also is applied to the
body on lesions in the treatment of seborrheic dermatitis or fungal infections.
(WHO) is discovering about medical
plants and traditional medicines and
exploitation of scientific validity of the herbal
medicines that has been used since
traditions. Herbal medicine
are great demand in
developing countries for primary
healthcare. There are some updated information on various
aspects of Lawsonia inermis a plant
that is used
all over the world that has been
compiled and review. This plant is known
as Henna or
Mhendi and live in tropical and subtropical areas. It plays appreciable role in Ayurvedic
and natural herbal medicines in ancient history of India. The archaeological evidence from Egypt that Lawsonia inermis (henna) used to
treat skin ailments and hair
colour five thousand years ago. There are
some evidence that brides marked their hands and feet with henna
for weddings in Mesapotamia and the
Eastern Mediterranean. In the 19th and early 20th
centuries, most of
the henna used
by Europe and
the USA was
grown in Egypt. Lawsonia inermis is biennial dicotyledonous herbaceous shrub which
in native of North Africa and South-
West Asia, the plant is
now widely cultivated through out the
tropics as an ornamental and dye
plant. In medicinally, the leaves
and aerial parts of Lawsonia inermis are frequently
used as herbal remedy for
human disorders such as
rheumatoid arthritis, headache, ulcers, diarrhea, leprosy, fever, leucorrhoea, diabetes, cardiac disease, hepatoprotective and as a coloring agent along
with other herbal medicines (Sarwat et al., 2012, Gul et al., 2012)
In this study, Lawsonia
inermis has demonstrated antibacterial activity against the bacterial
strains that have highest antibacterial activity against Pseudomonas Aeruginosa a species which is invasive and
toxigenic. Pseudomonas aeruginosa is a Gram-negative, aerobic bacterium that has been found
in water and soil. It is gastrointestinal tract of human beings and the skin is a normal flora. This bacterium is
an opportunistic pathogen which important microorganisms in nosocomial
infections in immune-compromised patients that have malignancies, cystic fibrosis and burns with
multiple pathogenic factors and high
rate of resistance towards antibiotics. This is because the presence of
glycocalyx in cellular membrane is responsible for binding of the bacterium to
the host cell easily, formation of biofilm and protection of bacteria against
penetration of antimicrobial agents and phagocytic
is the most commonly known as isolated nosocomial pathogen, where all acquired
infections about 10%. Hospitalized patients was increase up to 20% upon in the gastrointestinal carriage rates
due Pseudomonas aeruginosa in
immunocompromised patints with diabetes. Treatment for P.
aeruginosa infection is usually by intravenous combinations of
antibiotic but it doesn’t work. So, lawsonia
inermis (henna) can cure Peudomonas Aeruginosa infections.
Fresh henna leaves demonstrated the highest anti-microbial activity of P. aeruginosa . The inherent characteristics
of the fully grown plants is probably due to the maturity of its chemically
active constituents for example quinines.
The added presence of chlorophyll may be attributed in one of the
constituents of fresh leaves that are known to possess antimicrobial activity.
5% weight of the compound (2-hydroxy-1,4-naphthoquinone) contain in henna
leaves. These are the methods to determine anti- microbacterial activity by
henna against P.aeruginosa.
100g fresh leaves and dry leaves and 50g of fresh seeds and dry seeds are
soaked seperatelly in 500ml of ethanol
for 3 days. The mixture was filtered and extract the crude that has been
collected then heated at 48? in a water bath to evaporate its liquid content.
The dry crude henna was extracted and tested for its antimicrobacterial
activity. Although the fresh and dry seeds demonstrated antibacterial activity
against P. aeruginosa, our fresh henna leaves
demonstrated the highest anti-P. aeruginosa activity.
The most effective against the spectrum
of bacterial isolates tested is strikingly henna’s leaves as compared with its seeds. The two ketone substitutions contains aromatic
rings contain. They are characteristically highly active and ubiquitous in
nature. In addition, provide a source
with stable free radicals, to complex
irreversibly with nucleophilic amino acids in proteins whichly known as quinones . The antimicrobial effects is great on the
potential range of quinone. Lawsonia
Inermis has possess high antibacterial activities against P.
aeruginosa. Numerous free hydroxyl ions are capable to combine
with the carbohydrates and proteins during the antimicrobial activity in the
bacterial cell wall. The antibacterial activity of Lawsonia Inermis extraction
of alcoholic and oily were more effective results in a previous study.
Among all of the tested microorganisms, the methanolic extraction of L. inermis was more active against
S. aureus and Proteus sp. than others. These results are similar to that
obtained from a recent study. Other investigations showed that Henna is
effective against different microorganisms especially against P. aeruginosa. This clearly shown that lawsonia inermis can
cure skin infections by applying the extraction of henna’s leaves on the infenctinous skin.