Azithromycin method sufficiently sensitive. So, the developed method should

Azithromycin is an antibiotic and used for the bacterial infections. For the determination of azithromycin, an easy and isocratic RP-HPLC method was developed. This method was validated and used for the analysis of azithromycin in tablets, bulk samples and suspensions. The chromatographic conditions for the analysis were consisted of mobile phase which is mixture of 0.1 M KH2PO4 with acetonitrile and water. The C-18 column (250mm×4.6mm, 5mm) was used. The UV detector was used and the detection wavelength was set at 215nm. The sample injection volume was 20 ?L at flow rate of 1ml/min. The linearity was found to be (r2 = 0.997). The LOD was 78?g and LOQ was 20?g makes this method sufficiently sensitive. So, the developed method should be used for the routine laboratory analysis of samples. The HPTLC-densitometry method was used for the determination of clarithromycin, azithromycin, amodiaquine and artesunate. The sample and standard solution were prepared and wrapped with parafilm. The stationary phase were used in HPTLC-densitometry was silica gel60 F254. Different mobile phases have been applied such as for clarithromycin and azithromycin the mobile phase consists of methanol, ethyl acetate and concentrated ammonium hydroxide (20:5:0.5).For the determination of artesunate and amodiaquine the mobile phase consists of acetone-water-concentrated ammonium hydroxide (16:2.8:0.8). The UV detector was used which was operating at 254 nm. The correlation coefficient values of calibration curves were closer to 1. The estimation of azithromycin, clarithromycin, artesunate and amodiaquine was carried out by reagent free heating of layer which is called thermochemical activation which under 254 nm Uv light produce quench fluorescence. By this method some drugs which do not naturally quench fluorescence are also estimated by the silica gel layer activation. And this method is quiet secure and beneficial as compared to the chemical spray, dip or vapour phase reagent method. The estimation of azithromycin in pharmaceutical formulation was carried out by visible spectrophotometric method. The two methods which are method A and method B were used for this purpose. In method A, the secondary amine group which is present in azithromycin forms ion-association complex with the tropaeolineo-oo (TPoo) which have orange red coloured and absorption maximum at 490nm. In method B, secondary amine forms ion-association complex with alizarine red S (ARS) to formed yellow coloured whichDETERMINATION OF AZITHROMYCIN IN PHARMACEUTICAL PRODUCTS 2OSAMA NAVEED 2nd semester roll no 19shows absorption maximum at 440nm. Both the methods which are A and B obeys the beer’s law in the range from 5-25?g/ml. For this purpose the UV-visible spectrophotometer was used. The correlation coefficient for method A and method B were 0.9998 and 0.9997 respectively. In comparison with other methods such as HPLC and LC-MS this method is very easy with less cost and used for the azithromycin analysis in daily routine. The purpose of the present study is to establish a UV method for the determination of azithromycin and method should be definite, authentic and reliable.The double beam spectrophotometer with 1 cm quartz cells were used for spectral measurement. The phosphate buffer which has pH 6.8 was used as solvent for the preparation of stock and standard solutions of azithromycin. This method was also validated for various parameters such as specificity, accuracy, robustness, LOQ and LOD. The azithromycin UV spectrum shown that the maximum absorption was at 208nm. The correlation coefficient was come out to be 0.997 shows that the curve was linear in the range from 10-50?g/ml. The precision of the method depend upon the % RSD value so the values of % RSD was less than 2% shows the developed method is precise. The sensitivity of the method were related with the LOD and LOQ values and the LOD (1.6?g/ml) and LOQ (5?g/ml) shown the sensitivity of the method. The degradation of azithromycin was take place in the presence of alkaline, acidic and oxidation condition. Hence the results show that the developed method is selective, accurate, specific and sensitive. The developed method should be used for the determination of azithromycin in pharmaceutical products. The present work represents the method development and validation for the determination of azithromycin and cefpodoxime in pharmaceutical products.For the method development the double beam UV spectrophotometer which has wavelength range 190-1100nm, scan speed 600nm/min, 1cm quartz cells and band width 2nm was used. The validation of method takes place by using RP-HPLC technique. So the C-18 column (10mm×4.6mm, 5?m) was used for this purpose. The mobile phase consists of methanol, toluene: 100mM potassium dihydrogen phosphate buffer (60:30:10 v/v). The injection volume was 10?L and flow rate is 1ml/min. The validation of RP-HPLC method was depends upon factors such as linearity, accuracy, precision, specificity, lower limit of detection and lower limit of quantification. The correlation coefficient value for azithromycin was 0.9998 and for cefpodoxime was 0.9994 shows the linearity of the method. The precision of the method depend upon the % RSD value which is less than 2%. The accuracy was shownDETERMINATION OF AZITHROMYCIN IN PHARMACEUTICAL PRODUCTS 3OSAMA NAVEED 2nd semester roll no 19by % recovery which is 99.75. There is no interference and all peaks were separated shows the specificity of the method.The LOQ for azithromycin is found to be 0.50?g/ml and for cefpodoxime is 0.70?g/ml. The LOD for cefpodoxime and azithromycin were 0.320 ?g/ml and 0.250?g/ml respectively. The developed method is easy, very quick, very cheap and accurate and should be utilized for the determination of azithromycin in pharmaceutical products. The objective of the present study was to develop and validate HPLC and new spectrophotometric method for the determination of azithromycin and cefpodoxime in tablet dosage form. The uv-visible double beam spectrophotometer was used which have 1cm quartz cell 2nm spectral band width. The HPLC was consisting of C-18 column (150×4.6mm, 5?m) with an auto injection and uv detector. The mobile phase which was used is a mixture of methanol, acetonitrile and phosphate buffer (40:40:20 v/v). The ortho phosphoric acid was used to adjust the pH of mobile phase such as 6.5. The analyses were carried out at 20 0C and detection was carried out at 235nm. The flow rate of 1ml/min with 20?l injection volume was used.The validations were carried out by following parameters such as linearity, sensitivity, precision and robustness. The absorption maximum for the azithromycin was 232.4nm and for cefpodoxime was 218nm. The correlation coefficient value for cefpodoxime was 0.9984 and for azithromycin was 0.9978 over the concentration range of 20-100?g/ml for cefpodoxime and for azithromycin was 25-125?g/ml. So at the retention times of cefpodoxime and azithromycin there was no interfering peaks makes the HPLC more specific and selective. The % recovery shows that the method is very precise and accurate. The % RSD which is less than 2% makes this method more robust. The proposed method for the determination of cefpodoxime and azithromycin in tablets were accurate, sensitive and used for the routine analysis. In the medication of certain infections such as skin infection and infection in respiratory system, azithromycin has been used. The determination of azithromycin in pharmaceuticals dosage was carried out by high performance thin layer chromatography (HPTLC).To determine the azithromycin the HPTLC method has been established and validated. The stationary phase which was used is precoated silica gel 60F254 TLC plates (20×10 cm). The chloroform, ethanol and 25% ammonia were used as mobile phase. The samples and standards of azithromycin were spotted as narrow bands on TLC. The peak of azithromycin shows Rf value 0.54. The validation depends upon selectivity, linearity, accuracy, precision,DETERMINATION OF AZITHROMYCIN IN PHARMACEUTICAL PRODUCTS 4OSAMA NAVEED 2nd semester roll no 19LOQ and LOD. In spite, r2 = 0.993 of the simple regression model, its lack of fit was significant. The RSD % for the intermediate precision calculated from the percent recoveries results obtained by the simple linear model was found larger than required limit (2%). On the other hand, the quadratic model presented an r2 = 0.999 and an acceptable variance homogeneity. Based on the accuracy profile results, the simple regression model cannot be used as an adequate model. Applying a quadratic calibration model results in a situation where two-side 95%-tolerance interval for azithromycin falls within the acceptance limits for assays. Then, this approach is a suitable for assessing the content of azithromycin formulations by means of our HPTLC method for routine use. The major purpose of present study was to construct a colorimetric sensor for the estimation of azithromycin and this depend upon the reaction take place between unmodified silver nanoparticles and azithromycin. For the purpose of spectral determination the double beam spectrophotometer which have matched pair of 10mm quartz cells were used.The different parameters for the purpose are as follows: scan range 200-800nm, scan speed of 400nm/min and band width was 1nm. The IR spectrophotometer was used to record the FT-IR spectra in the range of 4000-400 cm-1. The silver nanoparticles were prepared and azithromycin solution was added in silver nanoparticles suspension and allowed to react for 5 min. Before the addition of azithromycin the nanoparticles shows SPR band at 394nm and with the addition of azithromycin the new SPR band occur at 500nm. The FT-IR spectra of silver nanoparticles shows stretching band of COO- at 1385 cm-1. When the azithromycin was added it shows the peak at 1392 cm-1. Due to the specific interaction between drug and silver nanoparticles the displacement of band takes place.The analytical conditions are pH of nanosuspension (3-6.7), incubation time (2.5 -20 min), concentration of azithromycin (0.2-100?M) and volume of silver nanoparticle suspension (1-3ml). From overall study it is evident that with good accuracy and precision this method was used successfully for the determination of azithromycin in pharmaceuticals. For the treatment of throat infection azithromycin sodium combined with cefixime are used. The main objective of the present work was to develop a new uv-spectrophotometric method which is used for the estimation of azithromycin and cefixime in tablets. For the determination purpose simultaneous equation method was used. For the absorption determination the uv-visible spectrophotometer which have quartz cell of 1cm path was used. The method validation was carried out by examining parameter such as accuracy, precision, LOQ and LOD. The common solvent which is used for both was methanol. The absorption maximumDETERMINATION OF AZITHROMYCIN IN PHARMACEUTICAL PRODUCTS 5OSAMA NAVEED 2nd semester roll no 19for azithromycin was 222nm and for cefixime was 289nm. The azithromycin LOD was 0.81?g/ml and for cefixime was 1.52?g/ml. for azithromycin the LOQ was 2.40?g/ml and for cefixime was 4.60?g/ml. The accuracy of this method was shown by recovery of azithromycin and cefixime which was 99.90% and 99.97% respectively. From the overall study and results it is concluded that this method of simultaneous equation were used for the determination of azithromycin and cefixime from tablets. The proposed method was used for the determination of macrolides and this is depend upon the formation of ion pair complex between macrolides with bromocresol green. The visible spectrophotometer was used for the spectral studies. The method was used for the estimation of macrolides such as azithromycin in pharmaceutical dosage and urine samples.The acetonitrile and ethanol was used as solvent. So all the three macrolides forms bluish-green coloured complex with bromocresol green and shows absorbance maxima 630nm for azithromycin, 620nm for roxithromycin and 625nm for erythromycin. So for wider linear range the bromocresol-green concentration of 40?M, 16?M and 20?M chosen respectively for azithromycin, roxithromycin and erythromycin. The correlation coefficient of calibration curve for azithromycin was 0.9990, for roxithromycin was 0.9975 and for erythromycin was 0.9997. The limit of detection was 0.19, 0.56 and 0.30 ?g/ml respectively. From the overall study it is shows that the method should be applied for the determination of these macrolides in pharmaceutical products. In the present work the purpose is to develop excellent method for the determination of cefixime and azithromycin in pharmaceutical formulations. For this purpose RP-HPLC method was used. The C-18 column was used with flow rate of 1ml/min with detection at 292nm. The parameter for the validation of develop method are accuracy, limit of detection, limit of quantification, linearity and precision. The retention time for both the drugs was found to be 2.8 for azithromycin and 3.9 for cefixime. The 1-5 ?g/ml is the range in which the detector response becomes linear for both drugs. The correlation coefficient for azithromycin was 0.9998 and for cefixime was 0.9996. The method was applied successfully for the simultaneous determination of azithromycin and cefixime in pharmaceutical formulation. The method was efficient, low cost, high speed and accurate and should be applied for routine analysis.